Composite

Part:BBa_K5520013:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)


pT7-LacO-His- CsnBK260Y


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 149
    Illegal NheI site found at 897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 641


Design Notes

We engineered a mutation by replacing the amino acid lysine (K) at position 260 with tyrosine (Y) to augment enzymatic activity and to yield more uniform products.


Source

Escherichia coli and Bacillus pumilus

References